By P. D. Bridge, D. K. Arora, C. A. Reddy, R. P. Elander
Polymerase chain response (PCR), a strong technique for amplifying DNA fragments, has had a major impression on learn all through biology. This publication discusses the wide-ranging functions of PCR in natural and utilized mycology.
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Additional info for Applications of PCR in Mycology
T. A. (1986) Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction. Cold Spring Harbor Symposia on Quantitative Biology 51, 263–273. W. H. (1991) Reverse transcription and DNA amplification by a Thermus thermophilus DNA polymerase. Biochemistry 30, 7661–7666. Nicholson, P. N. (1994) The use of random amplified polymorphic DNA to identify pathotype and detect variation in Pseudocercosporella herpotrichoides. Mycological Research 98, 13–21. J. (1994) PCR in situ Hybridization.
D. K. A. P. P. Goller et al. 1. 1 continued a Taq from Thermus aquaticus; Stoffel, a modified form of recombinant Taq DNA polymerase, with the N-terminal 289 amino acids containing the 5′→3′ exonuclease activity removed; Tth, from Thermus thermophilus; Pfu, from Pyrococcus furiosus; Vent from Thermococcus littoralis; Pwo from Pyrococcus woesei. , 1991; Hu, 1993; Biotechnology catalogue, Perkin Elmer, New Jersey, USA; Promega catalogue, Madison, Wisconsin, USA; Pfu DNA Polymerase Instruction Manual, Stratagene, La Jolla, California, USA, and references therein; data sheets for Vent DNA polymerase from NEB, New England, USA; Pwo DNA polymerase from Boehringer Mannheim, Germany).
PCR-based methods offer so many possibilities that they are now used in almost all areas of life sciences. In mycology, many applications have already been described in taxonomy, phylogeny, population studies and diagnostics, and some examples have been reported in this chapter. However, PCR applications are being developed in many other fungal studies, including genetic analyses, biotechnology and the analysis of fungal–host interactions in both medical and plant pathology. The following chapters provide current reviews of many research applications of PCR in all these areas of mycology.
Applications of PCR in Mycology by P. D. Bridge, D. K. Arora, C. A. Reddy, R. P. Elander